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KMID : 1098420060140030125
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 3 p.125 ~ p.129
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Genetic Analysis of Polygonati Rhizoma and Polygonati odorati Rhizoma using Random Amplified Microsatellite Polymorphism
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An Sun-Min
Ryuk Jin-Ah
Kim Young-Hwa
Chae Byoung-Chan
Kim Hong-Jun
Kim Ki-Hoon
Kang Kwon-Kyoo
Ko Byoung-Seob
Lee Mi-Young
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Abstract
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Two herbal medicines of the Polygonatum genus, namely Polygonati Rhizoma and Polygonati Odorati Rhizoma, are difficult to distinguish from each other through exterior morphologic aspects. Furthermore, because the standard components for physiochemical distinction have not yet been standardized, the identification of these medicines through botanic taxology is based on genetic methods of random amplified microsatellite polymorphism (RAMP). For the RAMP evaluation, we used five sets of UBC microsatellite primers 811, 818, 834, 836, 842 and random primer M1. Although no specific band that could clearly distinguish Polygonati Rhizoma from Polygonati Odorati Rhizoma was found, 11 Polygonatum plants could be divided into two groups by this method. P. sibiricum and P. stenophyllum were classified to group I and the others were to group II. As P. sibiricum and P. stenophyllum were very similar in genetic and morphologic perspective, the results suggest that P. stenophyllum belongs to the Polygonati Rhizoma family.
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KEYWORD
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Polygonati Rhizoma, Polygonati Odorati Rhizoma, RAMP
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